Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Reduced replication of human immunodeficiency virus type 1 mutants that use reverse transcription primers other than the natural tRNA(3Lys).

Journal of virology - 1 May 1995

Das A T, Klaver B, Berkhout B

Abstract excerpt

Replication of the human immunodeficiency virus type 1 (HIV-1) and other retroviruses involves reverse transcription of the viral RNA genome into a double-stranded DNA. This reaction is primed by the cellular tRNA(3Lys) molecule, which binds to a complementary sequence in the viral genome, referred to as the primer-binding site (PBS). In order to study the specificity of primer usage, we constructed a set of...

Topics

Base Sequence
Binding Sites
DNA Primers
DNA, Viral
HIV-1
HeLa Cells
Humans
Molecular Sequence Data
Mutation
RNA, Transfer, Amino Acid-Specific
RNA, Transfer, Lys

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Viral

Transcription, Genetic

Transfection

Virus Replication

Source

Open in PubMed Open full text

Identifiers

PMID: 7707537
DOI: 10.1128/JVI.69.5.3090-3097.1995
PMCID: PMC189010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7707537-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Reduced replication of human immunodeficiency virus type 1 mutants that use reverse transcription primers other than the natural tRNA(3Lys).PMID 7707537Read ↗

Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replicationSemantic proximity Read ↗

The tRNA primer activation signal in the human immunodeficiency virus type 1 genome is important for initiation and processive elongation of reverse transcription.Semantic proximity Read ↗

A structured RNA motif is involved in correct placement of the tRNA(3)(Lys) primer onto the human immunodeficiency virus genome.Semantic proximity Read ↗

Impact of forced selection of tRNAs on HIV-1 replication and genome stability highlight preferences for selection of certain tRNAs.Semantic proximity Read ↗

Forced selection of tRNA(Glu) reveals the importance of two adenosine-rich RNA loops within the U5-PBS for SIV(smmPBj) replication.Semantic proximity Read ↗

HIV type 1 that select tRNA(His) or tRNA(Lys1,2) as primers for reverse transcription exhibit different infectivities in peripheral blood mononuclear cells.Semantic proximity Read ↗

Mutations within the primer binding site of the human immunodeficiency virus type 1 define sequence requirements essential for reverse transcription.Semantic proximity Read ↗

Switching the in vitro tRNA usage of HIV-1 by simultaneous adaptation of the PBS and PAS.Semantic proximity Read ↗

Deletions in the tRNA(Lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcriptionSemantic proximity Read ↗

Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells.Semantic proximity Read ↗

New insights into inhibition of human immunodeficiency virus type 1 replication through mutant tRNALys3.Semantic proximity Read ↗

Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.