A Real-Time, Programmable, and Multiplex Ligation Chain Reaction-MNAzyme Platform for Single-Nucleotide Variant Detection.

Abstract excerpt

Here, we present a modular and programmable real-time quantitative detection platform that integrates ligase chain reaction (LCR) with multicomponent nucleic acid enzymes (MNAzymes) for single-nucleotide variant (SNV) analysis. By incorporating a universal MNAzyme-based reporter, the LCR-MNAzyme system enables PCR-like real-time quantitative monitoring of SNVs while supporting programmable and multiplex...

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• JAK2 gene
• Janus Kinase 2
• Limit of Detection
• Multiplex Polymerase Chain Reaction
• Polymorphism, Single Nucleotide