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Article

LacI-mediated sequence-specific affinity purification of plasmid DNA for therapeutic applications.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology - 1 May 2005

Darby Richard A J, Hine Anna V

Abstract excerpt

Affinity purification of plasmid DNA is an attractive option for the biomanufacture of therapeutic plasmids, which are strictly controlled for levels of host protein, DNA, RNA, and endotoxin. Plasmid vectors are considered to be a safer alternative than viruses for gene therapy, but milligram quantities of DNA are required per dose. Previous affinity approaches have involved triplex DNA formation and a...

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PMID: 15760969
DOI: 10.1096/fj.04-2812fje

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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