Article

miniVec: A miniaturized plasmid backbone supporting antibiotic-free and additive-free fermentation with enhanced yield and functionality

2025-10-03

Abstract excerpt

<h4>ABSTRACT</h4> Plasmids are a foundational research reagent and a key material in biopharmaceutical manufacturing. Plasmids require a backbone for propagation in E. coli, which typically contains an antibiotic resistance gene alongside a replication origin. As plasmids increasingly enter clinical applications, concerns are raised on the safety risks of antibiotic resistance genes. Additionally, these protein-c...

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Literature Corpus work: 6bdede2d-9192-52ba-a368-685921cf52ee
DOI: 10.1101/2025.10.02.679775

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