Analysis of expressed N-ras mutations in human melanoma short-term cell lines with allele specific restriction analysis induced by the polymerase chain reaction.

Abstract excerpt

Mutations in ras genes have been found in the DNA of numerous cancer types including melanomas, but the expression of these mutations in melanomas has not yet been addressed. We have used the polymerase chain reaction (PCR) and allele-specific restriction analysis (ASRA) to determine the frequency of expressed N-ras mutations on 25 short-term melanoma tissue culture samples. N-ras cDNA generated using reverse...

Topics

• Alleles
• Base Sequence
• Cell Line
• Genes, ras
• Humans
• Melanoma
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction
• Skin Neoplasms