Article
Allele-specific detection of K-ras oncogene expression in human non-small-cell lung carcinomas.
International journal of cancer - 22 Apr 1991
Slebos R J, Habets G G, Evers S G, Mooi W J, Rodenhuis S
Abstract excerpt
Point mutations in codon 12 of the K-ras oncogene are frequent in human lung adenocarcinomas. To study the expression of the K-ras gene in these tumors we have developed a mRNA detection technique based on the polymerase chain reaction (PCR). By this technique, K-ras expression can be detected se...
Topics
- Adenocarcinoma
- Alleles
- Base Sequence
- Carcinoma
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Squamous Cell
- Cell Line
- Colonic Neoplasms
- DNA, Neoplasm
- Gene Expression
- Genes, ras
- Humans
- Immunoblotting
- Lung Neoplasms
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction