Article
The effect of nitrogen source on yield and glycosylation of a human cystatin C mutant expressed in Pichia pastoris.
Journal of industrial microbiology & biotechnology - 1 Dec 2004
Pritchett Jason, Baldwin Susan A
Abstract excerpt
Human cystatin C is a cysteine proteinase inhibitor with potential applications as an anti-viral agent, cancer tumor growth inhibitor, and in prevention of proteolysis during food processing. A glycosylated cystatin C mutant with increased temperature stability was developed for the latter application [Nakamura et al. (1998) FEBS Lett 427:252-254]. A recombinant variant of cystatin C [Nakamura et al. (2000)...
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