Article
Production of human salivary type cysteine proteinase inhibitors (cystatins) by an Escherichia coli system and partial characterization of recombinant cystatin S and its mutant (117 arginine-->tryptophan).
Journal of biochemistry - 1 Aug 1994
Saitoh E, Isemura S
Abstract excerpt
The cDNAs encoding the precursors of cystatin SN, cystatin S, and two mutants of cystatin S (-18R-->W; 117R-->W) were expressed in Escherichia coli JM109 with isopropyl-beta-D-thio-galactoside (IPTG) induction. Premature cystatin S with the original signal [-20MARPLCTLLLLMATLAGALA] was processed...
Topics
- Amino Acid Sequence
- Arginine
- Base Sequence
- Cystatins
- DNA, Complementary
- Escherichia coli
- Genetic Vectors
- Humans
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Salivary Cystatins
- Tryptophan