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Article

Production, inhibitory activity, folding and conformational analysis of an N-terminal and an internal deletion variant of chicken cystatin.

European journal of biochemistry - 1 Sept 1994

Auerswald E A, Nägler D K, Schulze A J, Engh R A, Genenger G, Machleidt W, Fritz H

Abstract excerpt

Two deletion variants of chicken cystatin were produced after cassette mutagenesis of the recombinant Arg-Glu-Phe-[Met1, Ile29, Leu89]-chicken egg white cystatin gene in Escherichia coli. The variant des-Ser1-Pro11-[Ala12, Glu13, Phe14, Met15, Ile29, Leu89]-chicken cystatin (N-del 2) and the variant Arg-Glu-Phe-[Met1, Ile29]-des-Cys71-Met89-chicken cystatin (del-helix II) were purified and characterized by...

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PMID: 7925354
DOI: 10.1111/j.1432-1033.1994.00407.x

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