Production, inhibitory activity, folding and conformational analysis of an N-terminal and an internal deletion variant of chicken cystatin.

doi:10.1111/j.1432-1033.1994.00407.x

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Article

Production, inhibitory activity, folding and conformational analysis of an N-terminal and an internal deletion variant of chicken cystatin.

European journal of biochemistry - 1 Sept 1994

Auerswald E A, Nägler D K, Schulze A J, Engh R A, Genenger G, Machleidt W, Fritz H

Abstract excerpt

Two deletion variants of chicken cystatin were produced after cassette mutagenesis of the recombinant Arg-Glu-Phe-[Met1, Ile29, Leu89]-chicken egg white cystatin gene in Escherichia coli. The variant des-Ser1-Pro11-[Ala12, Glu13, Phe14, Met15, Ile29, Leu89]-chicken cystatin (N-del 2) and the vari...

Topics

Amino Acid Sequence
Animals
Base Sequence
Chickens
Circular Dichroism
Cloning, Molecular
Cystatins
Cysteine Endopeptidases
Genetic Variation
Guanidine
Guanidines
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Insertional

Oligodeoxyribonucleotides

Protein Conformation

Protein Denaturation

Protein Folding

Protein Structure, Secondary

Source

Open in PubMed

Identifiers

PMID: 7925354
DOI: 10.1111/j.1432-1033.1994.00407.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7925354-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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