Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization of the wheat puroindoline-a production in Pichia pastoris

2 Mar 2001

Abstract excerpt

AIMS: A recombinant puroindoline-a (rPIN-a) was produced using the methylotrophic yeast Pichia pastoris. METHODS AND RESULTS: In fed-batch culture, the production of rPIN-a decreased after 24 h of methanol induction. Most of the rPIN-a was not soluble in the culture medium remaining bound to the cell walls. Soluble and membrane-bound rPIN-a were quantified by ELISA after Triton X-114 phase partitioning. In order...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biochemical and Structural Characterization
Share to Fungal and yeast genetics research
Share to Peptidase Inhibition and Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 11298235
DOI: 10.1046/j.1365-2672.2001.01259.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization of the wheat puroindoline-a production in Pichia pastorisPMID 11298235Read ↗

Recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

Production of bioactive wheat puroindoline proteins in Nicotiana benthamiana using a virus-based transient expression system.Semantic proximity Read ↗

Expression, purification and antimicrobial activity of puroindoline A protein and its mutants.Semantic proximity Read ↗

Strategies to maximize expression of rightly processed human interferon α2b in Pichia pastorisSemantic proximity Read ↗

Recombinant production of the human aquaporins in the yeast<i>Pichia pastoris</i>(Invited Review)Semantic proximity Read ↗

Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose HostSemantic proximity Read ↗

Evaluation of Mut(S) and Mut⁺ Pichia pastoris strains for membrane-bound catechol-O-methyltransferase biosynthesis.Semantic proximity Read ↗

New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris.Semantic proximity Read ↗

Process engineering of biopharmaceutical production in moss bioreactors via model-based description and evaluation of phytohormone impactSemantic proximity Read ↗

Advances in the use of Pichia pastoris for high-level gene expressionSemantic proximity Read ↗

High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability.Semantic proximity Read ↗

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.