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Efficient mutagenesis method for producing the templates of single nucleotide polymorphisms.

Molecular biotechnology - 1 Jun 2003

Zhang Jia, Li Kai, Deng Zemin, Liao Duanfang, Fang Weiyi, Zhang Xu

Abstract excerpt

DNA templates harboring specific single nucleotide polymorphism (SNP) sites are largely needed as positive controls in practical SNP analysis and in determination of the reliability of newly developed methods in high-throughput screening assays. Here we report a one-step method to produce SNP templates by amplifying a wild-type sequence with primers having single nucleotide mismatches at or near their 3' ends. A...

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PMID: 12746551
DOI: 10.1385/MB:24:2:105

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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