Article
Assessing genetic heterogeneity in production cell lines: detection by peptide mapping of a low level Tyr to Gln sequence variant in a recombinant antibody.
Bio/technology (Nature Publishing Company) - 1 Nov 1993
Harris R J, Murnane A A, Utter S L, Wagner K L, Cox E T, Polastri G D, Helder J C, Sliwkowski M B
Abstract excerpt
A recombinant antibody directed against the human epidermal growth factor receptor-2 extracellular domain was subjected to detailed structural characterization. Heterogeneity in the heavy chain was demonstrated by recovery of two forms of a tryptic peptide, with either glutamine or the expected tyrosine at residue 376. Subsequent experiments indicated that the Y376Q variant developed during transfection of the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- CHO Cells
- Cell Line
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Cricetinae
- Culture Techniques
- DNA Primers