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Article

Design and construction of highly stable, protease-resistant chimeric avidins.

The Journal of biological chemistry - 18 Mar 2005

Hytönen Vesa P, Määttä Juha A E, Nyholm Thomas K M, Livnah Oded, Eisenberg-Domovich Yael, Hyre David, Nordlund Henri R, Hörhä Jarno, Niskanen Einari A, Paldanius Tiina, Kulomaa Tuomas, Porkka Eevaleena J, Stayton Patrick S, Laitinen Olli H, Kulomaa Markku S

Abstract excerpt

The chicken avidin gene family consists of avidin and seven separate avidin-related genes (AVRs) 1-7. Avidin protein is a widely used biochemical tool, whereas the other family members have only recently been produced as recombinant proteins and characterized. In our previous study, AVR4 was found to be the most stable biotin binding protein thus far characterized (T(m) = 106.4 degrees C). In this study, we...

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PMID: 15649900
DOI: 10.1074/jbc.M414196200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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