Article
Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences.
The Biochemical journal - 15 Jan 2003
Marttila Ari T, Hytönen Vesa P, Laitinen Olli H, Bayer Edward A, Wilchek Meir, Kulomaa Markku S
Abstract excerpt
The strong interaction between avidin and biotin is so tight (dissociation constant 10(-15) M) that conditions usually sufficient for protein denaturing fail to dislodge biotin from the avidin-biotin complex. This kind of irreversible binding hinders the use of avidin in applications such as affinity purification or protein immobilization. To address this concern, we have constructed a series of mutants of the...
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