Article
Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces.
The Journal of biological chemistry - 24 Jan 2003
Nordlund Henri R, Laitinen Olli H, Uotila Sanna T H, Nyholm Thomas, Hytönen Vesa P, Slotte J Peter, Kulomaa Markku S
Abstract excerpt
In this study we showed that tetrameric chicken avidin can be stabilized by introducing intermonomeric disulfide bridges between its subunits. These covalent bonds had no major effects on the biotin binding properties of the respective mutants. Moreover, one of the mutants (Avd-ccci) maintained its tetrameric integrity even in denaturing conditions. The new avidin forms Avd-ci and Avd-ccci, which have native -->...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.