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Article

Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins.

The Journal of biological chemistry - 27 Aug 2004

Nordlund Henri R, Laitinen Olli H, Hytönen Vesa P, Uotila Sanna T H, Porkka Eevaleena, Kulomaa Markku S

Abstract excerpt

Two distinct circularly permuted forms of chicken avidin were designed with the aim of constructing a fusion avidin containing two biotin-binding sites in one polypeptide. The old N and C termini of wild-type avidin were connected to each other via a glycine/serine-rich linker, and the new termini were introduced into two different loops. This enabled the creation of the desired fusion construct using a short...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 15131113
DOI: 10.1074/jbc.M403496200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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