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Article

Site-specific labeling of the ribosome for single-molecule spectroscopy.

Nucleic acids research - 1 Jan 2005

Dorywalska Magdalena, Blanchard Scott C, Gonzalez Ruben L, Kim Harold D, Chu Steven, Puglisi Joseph D

Abstract excerpt

Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach requires site-specific and stable attachment of fluorophores to the components of translation machinery. Fluorescent tagging of the ribosome is a prerequisite for the observation of dynamic changes in ribosomal...

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Identifiers

PMID: 15647501
DOI: 10.1093/nar/gki151
PMCID: PMC546139

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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