Article
EF-G catalyzed translocation dynamics in the presence of ribosomal frameshifting stimulatory signals.
Nucleic acids research - 17 Mar 2017
Kim Hee-Kyung, Tinoco Ignacio
Abstract excerpt
Programmed -1 ribosomal frameshifting (-1PRF) is tightly regulated by messenger RNA (mRNA) sequences and structures in expressing two or more proteins with precise ratios from a single mRNA. Using single-molecule fluorescence resonance energy transfer (smFRET) between (Cy5)EF-G and (Cy3)tRNALys, we studied the translational elongation dynamics of -1PRF in the Escherichia coli dnaX gene, which contains three...
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