Article
Directed evolution of rRNA improves translation kinetics and recombinant protein yield.
Nature communications - 24 Sept 2021
Liu Fan, Bratulić Siniša, Costello Alan, Miettinen Teemu P, Badran Ahmed H
Abstract excerpt
In bacteria, ribosome kinetics are considered rate-limiting for protein synthesis and cell growth. Enhanced ribosome kinetics may augment bacterial growth and biomanufacturing through improvements to overall protein yield, but whether this can be achieved by ribosome-specific modifications remains unknown. Here, we evolve 16S ribosomal RNAs (rRNAs) from Escherichia coli, Pseudomonas aeruginosa, and Vibrio...
Topics
- Base Sequence
- Directed Molecular Evolution
- Escherichia coli
- Kinetics
- Mass Spectrometry
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- Protein Biosynthesis
- Proteome
- RNA, Ribosomal
- RNA, Ribosomal, 16S
- Recombinant Proteins
- Ribosomal Proteins