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Article

Directed evolution of rRNA improves translation kinetics and recombinant protein yield.

Nature communications - 24 Sept 2021

Liu Fan, Bratulić Siniša, Costello Alan, Miettinen Teemu P, Badran Ahmed H

Abstract excerpt

In bacteria, ribosome kinetics are considered rate-limiting for protein synthesis and cell growth. Enhanced ribosome kinetics may augment bacterial growth and biomanufacturing through improvements to overall protein yield, but whether this can be achieved by ribosome-specific modifications remains unknown. Here, we evolve 16S ribosomal RNAs (rRNAs) from Escherichia coli, Pseudomonas aeruginosa, and Vibrio...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 34561441
DOI: 10.1038/s41467-021-25852-5
PMCID: PMC8463689

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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