Article
Opposing roles for actin in Cdc42p polarization.
Molecular biology of the cell - 1 Mar 2005
Irazoqui Javier E, Howell Audrey S, Theesfeld Chandra L, Lew Daniel J
Abstract excerpt
In animal and fungal cells, the monomeric GTPase Cdc42p is a key regulator of cell polarity that itself exhibits a polarized distribution in asymmetric cells. Previous work showed that in budding yeast, Cdc42p polarization is unaffected by depolymerization of the actin cytoskeleton (Ayscough et al., J. Cell Biol. 137, 399-416, 1997). Surprisingly, we now report that unlike complete actin depolymerization, partial...
Topics
- Actins
- Binding Sites
- Cytoskeleton
- Endocytosis
- Genotype
- Microscopy, Fluorescence
- Models, Biological
- Mutation
- Protein Binding
- Saccharomyces cerevisiae
- Temperature
- Time Factors
- cdc42 GTP-Binding Protein