Article
Saccharomyces cerevisiae Cdc42p localizes to cellular membranes and clusters at sites of polarized growth.
Eukaryotic cell - 1 Jun 2002
Richman Tamara J, Sawyer Mathew M, Johnson Douglas I
Abstract excerpt
The Cdc42p GTPase controls polarized growth and cell cycle progression in eukaryotes from yeasts to mammals, and its precise subcellular localization is essential for its function. To examine the cell cycle-specific targeting of Cdc42p in living yeast cells, a green fluorescent protein (GFP)-Cdc42 fusion protein was used. In contrast to previous immunolocalization data, GFP-Cdc42p was found at the plasma membrane...
Topics
- Actomyosin
- Amino Acid Sequence
- Cell Division
- Cell Membrane
- Cell Polarity
- Green Fluorescent Proteins
- Intracellular Membranes
- Luminescent Proteins
- Models, Biological
- Mutation
- Protein Structure, Tertiary
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- cdc42 GTP-Binding Protein, Saccharomyces cerevisiae