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Article

Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse Probe System in challenging sequence environment.

Human genomics - 1 Mar 2004

Belousov Yevgeniy S, Welch Robert A, Sanders Silvia, Mills Alan, Kulchenko Alena, Dempcy Robert, Afonina Irina A, Walburger David K, Glaser Cynthia L, Yadavalli Sunita, Vermeulen Nicolaas M J, Mahoney Walt

Abstract excerpt

Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to lower GC-content sequences only. We have developed the MGB Eclipse Probe System, which is composed of the following elements: MGB Eclipse probes and primers, specially...

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Identifiers

PMID: 15588480
DOI: 10.1186/1479-7364-1-3-209
PMCID: PMC3525082

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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