Article
The allele-specific probe and primer amplification assay, a new real-time PCR method for fine quantification of single-nucleotide polymorphisms in pooled DNA.
Applied and environmental microbiology - 1 Feb 2012
Billard A, Laval V, Fillinger S, Leroux P, Lachaise H, Beffa R, Debieu D
Abstract excerpt
The evolution of fungicide resistance within populations of plant pathogens must be monitored to develop management strategies. Such monitoring often is based on microbiological tests, such as microtiter plate assays. Molecular monitoring methods can be considered if the mutations responsible for resistance have been identified. Allele-specific real-time PCR approaches, such as amplification refractory mutation...
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