Article
[Localization of human telomere repeat binding factor 1 in telomerase-positive and-negative cells and its expression during cell cycle].
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 1 Nov 2004
Lan Jian-ping, La Xiao-yu, Zhu Yuan-yuan, Sun Jie, Li Jing-yuan, Yu Jian, Tan Ya-min, Shi Ji-min, Lin Mao-fang, Huang He
Abstract excerpt
OBJECTIVE: To observe the distribution pattern of human telomere repeat binding factor 1(TRF1) in the telomerase-positive (HeLa) and telomerase-negative cells (WI38-2RA) and to investigate its expression level during the cell cycle. METHODS: The full-length sequences of TRF1(TRF1FL) and its mutant with N and C terminus deletion (TRF1DeltaNC) were generated by PCR amplification, the resulting fragments were cloned...
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