Article
Biased mutation-assembling: an efficient method for rapid directed evolution through simultaneous mutation accumulation.
Protein engineering, design & selection : PEDS - 1 Jun 2005
Hamamatsu Norio, Aita Takuyo, Nomiya Yukiko, Uchiyama Hidefumi, Nakajima Motowo, Husimi Yuzuru, Shibanaka Yasuhiko
Abstract excerpt
We have developed an efficient optimization technique, 'biased mutation-assembling', for improving protein properties such as thermostability. In this strategy, a mutant library is constructed using the overlap extension polymerase chain reaction technique with DNA fragments from wild-type and phenotypically advantageous mutant genes, in which the number of mutations assembled in the wild-type gene is...
Topics
- Chryseobacterium
- Directed Molecular Evolution
- Gene Expression
- Gene Library
- Genetic Vectors
- Hot Temperature
- Mutation
- Prolyl Oligopeptidases
- Protein Engineering
- Serine Endopeptidases