A biochemically defined system for mammalian nonhomologous DNA end joining.

doi:10.1016/j.molcel.2004.11.017

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Article

A biochemically defined system for mammalian nonhomologous DNA end joining.

Molecular cell - 3 Dec 2004

Ma Yunmei, Lu Haihui, Tippin Brigette, Goodman Myron F, Shimazaki Noriko, Koiwai Osamu, Hsieh Chih-Lin, Schwarz Klaus, Lieber Michael R

Abstract excerpt

Nonhomologous end joining (NHEJ) is a major pathway in multicellular eukaryotes for repairing double-strand DNA breaks (DSBs). Here, the NHEJ reactions have been reconstituted in vitro by using purified Ku, DNA-PK(cs), Artemis, and XRCC4:DNA ligase IV proteins to join incompatible ends to yield diverse junctions. Purified DNA polymerase (pol) X family members (pol mu, pol lambda, and TdT, but not pol beta)...

Topics

Animals
Antigens, Nuclear
Base Sequence
DNA
DNA Damage
DNA Repair
DNA-Activated Protein Kinase
DNA-Binding Proteins
DNA-Directed DNA Polymerase
Endonucleases
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Ku Autoantigen

Models, Biological

Models, Genetic

Molecular Sequence Data

Mutation

Nuclear Proteins

Oligonucleotides

Source

Open in PubMed

Identifiers

PMID: 15574326
DOI: 10.1016/j.molcel.2004.11.017

Genes

XRCC4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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