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Article

Evolution of new nonantibody proteins via iterative somatic hypermutation.

Proceedings of the National Academy of Sciences of the United States of America - 30 Nov 2004

Wang Lei, Jackson W Coyt, Steinbach Paul A, Tsien Roger Y

Abstract excerpt

B lymphocytes use somatic hypermutation (SHM) to optimize immunoglobulins. Although SHM can rescue single point mutations deliberately introduced into nonimmunoglobulin genes, such experiments do not show whether SHM can efficiently evolve challenging novel phenotypes requiring multiple unforeseeable mutations in nonantibody proteins. We have now iterated SHM over 23 rounds of fluorescence-activated cell sorting...

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Identifiers

PMID: 15556995
DOI: 10.1073/pnas.0407752101
PMCID: PMC529417

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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