Directed evolution of mammalian anti-apoptosis proteins by somatic hypermutation.

doi:10.1093/protein/gzr052

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Article

Directed evolution of mammalian anti-apoptosis proteins by somatic hypermutation.

Protein engineering, design & selection : PEDS - 1 Jan 2012

Majors Brian S, Chiang Gisela G, Pederson Nels E, Betenbaugh Michael J

Abstract excerpt

Recently, researchers have created novel fluorescent proteins by harnessing the somatic hypermutation ability of B cells. In this study, we examined if this approach could be used to evolve a non-fluorescent protein, namely the anti-apoptosis protein Bcl-x(L), using the Ramos B-cell line. After demonstrating that Ramos cells were capable of mutating a heterologous bcl-x(L) transgene, the cells were exposed to...

Topics

Amino Acid Sequence
Animals
Apoptosis
Apoptosis Regulatory Proteins
B-Lymphocytes
Base Sequence
Blotting, Western
CHO Cells
Caspase 3
Cell Line, Tumor
Cell Survival

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Cricetinae

Cricetulus

Directed Molecular Evolution

Enzyme Inhibitors

Humans

Luminescent Proteins

Mammals

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Identifiers

PMID: 22160868
DOI: 10.1093/protein/gzr052
PMCID: PMC3276308

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