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Optimised nanobody-based quenchbodies for enhanced protein detection

18 Jun 2025

Abstract excerpt

Quenchbodies, antibodies labelled with fluorophores that increase in intensity upon antigen binding, offer great promise for biosensor development. Nanobody-based quenchbodies are particularly attractive due to their small size, ease of expression, high stability, rapid evolvability, and amenability to protein engineering. However, existing designs for protein detection show limited dynamic range, with...

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PMID: 40533519
DOI: 10.1038/s42003-025-08359-3

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