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Article

Development of a tightly regulated U6 promoter for shRNA expression.

FEBS letters - 19 Nov 2004

Lin Xiaoyu, Yang Jianguo, Chen Jun, Gunasekera Angelo, Fesik Stephen W, Shen Yu

Abstract excerpt

Short hairpin RNAs (shRNAs) have been used to achieve stable target knockdown in a variety of biological systems. Here, we report the development of a tightly regulated tetracycline-responsive human U6 promoter for shRNA expression. By engineering two copies of the tet operators flanking the TATA box of the human U6 promoter, we created a U6 promoter derivative (2O2) that exhibited much lower basal...

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PMID: 15556613
DOI: 10.1016/j.febslet.2004.10.033

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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