Article

Exploring the use of alternative promoters for enhanced transgene and sgRNA expression in Atlantic salmon cells

2024-02-01

Abstract excerpt

This study facilitates design of expression vectors and lentivirus tools for gene editing of Atlantic salmon. We have characterized widely used heterologous promoters and novel endogenous promoters in Atlantic salmon cells. We used qPCR to evaluate the activity of several U6 promoters for sgRNA expression, including human U6 (hU6), tilapia U6 (tU6), mouse U6 (mU6), zebrafish U6 (zU6), Atlantic salmon U6 (sU6), med...

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Literature Corpus work: 36359d74-cc24-5785-9c41-61f841c47323
DOI: 10.21203/rs.3.rs-3899646/v1

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