Article
Induction and progression of the transformed phenotype in cloned rat embryo fibroblast cells: studies employing type 5 adenovirus and wild-type and mutant Ha-ras oncogenes.
Molecular carcinogenesis - 1 Jan 1992
Boylan J F, Shih T Y, Fisher P B, Zimmer S G
Abstract excerpt
Transformation of cloned rat embryo fibroblast (CREF) cells with the wild-type 5 adenovirus (wtAd5) transforming genes E1A and E1B (which extend from 0 to 11.2 map units) results in morphologically transformed cells that exhibit an increased saturation density in monolayer culture and display an anchorage-independent phenotype. WtAd5-transformed CREF (wtAd5 CREF) cells do not, however, induce tumors when injected...
Topics
- Adenoviridae
- Animals
- Cell Line
- Cell Transformation, Viral
- Clone Cells
- Embryo, Mammalian
- Female
- Fibroblasts
- Genes, ras
- Mice
- Mice, Nude