Article
Mutation of H-ras is infrequent in bladder cancer: confirmation by single-strand conformation polymorphism analysis, designed restriction fragment length polymorphisms, and direct sequencing.
Cancer research - 1 Jan 1993
Knowles M A, Williamson M
Abstract excerpt
A series of 152 human bladder tumors, 14 bladder tumor cell lines, and 1 immortal urothelial cell line were examined by single-strand conformation polymorphism (SSCP) and designed restriction fragment.length polymorphism analyses for mutations in exons 1 and 2 of the H-ras gene. Nine tumors (6%)...
Topics
- Base Sequence
- Codon
- DNA, Single-Stranded
- Electrophoresis, Polyacrylamide Gel
- Exons
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Neoplasm Staging
- Nucleic Acid Conformation
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Reproducibility of Results
- Sequence Analysis, DNA
- Urinary Bladder Neoplasms