Article
Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.
Journal of bacteriology - 1 Nov 1990
Herrero M, de Lorenzo V, Timmis K N
Abstract excerpt
A simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features of Tn10 and Tn5; (ii) the resistances to the herbicide bialaphos, to mercuric salts and organomer...
Topics
- Anti-Bacterial Agents
- Arsenic
- Arsenites
- Base Sequence
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- Drug Resistance, Microbial
- Escherichia coli
- Genetic Vectors
- Genotype
- Gram-Negative Bacteria