Random Mutagenesis Using Error-Prone DNA Polymerases.

doi:10.1101/pdb.prot097741

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Article

Random Mutagenesis Using Error-Prone DNA Polymerases.

Cold Spring Harbor protocols - 1 Mar 2018

Forloni Matteo, Liu Alex Y, Wajapeyee Narendra

Abstract excerpt

"Random mutagenesis" is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence. Once developed, these libraries can then be used for several purposes, including structure-function and directed evolution studies. Random mutagenesis is different from other mutagenesis techniques in that it does not require the researcher to have any prior knowledge about the...

Topics

Cloning, Molecular
DNA
DNA Replication
DNA-Directed DNA Polymerase
Gene Library
Humans
Mutagenesis
Mutation
Plasmids
Templates, Genetic

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Identifiers

PMID: 29496818
DOI: 10.1101/pdb.prot097741

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