Article
Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Shao Weilan, Ma Kesen, Le Yilin, Wang Hongcheng, Sha Chong
Abstract excerpt
Directed evolution methods are increasingly needed to improve gene and protein properties. Error-prone PCR is the most efficient method to introduce random mutations by reducing the fidelity of the DNA polymerase. However, a highly efficient process is required for constructing and screening a diverse mutagenesis library since a large pool of transformants is needed to generate a desired mutant. We developed a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.