Article
The topology of the Lcb1p subunit of yeast serine palmitoyltransferase.
The Journal of biological chemistry - 17 Dec 2004
Han Gongshe, Gable Ken, Yan Lianying, Natarajan Mukil, Krishnamurthy Jayasree, Gupta Sita D, Borovitskaya Anna, Harmon Jeffrey M, Dunn Teresa M
Abstract excerpt
The structural organization and topology of the Lcb1p subunit of yeast and mammalian serine palmitoyltransferases (SPT) were investigated. In the yeast protein, three membrane-spanning domains were identified by insertion of glycosylation and factor Xa cleavage sites at various positions. The first domain of the yeast protein, located between residues 50 and 84, was not required for the stability, membrane...
Topics
- Acyltransferases
- Alleles
- Amino Acid Sequence
- Animals
- Binding Sites
- Blotting, Western
- CHO Cells
- Cell Membrane
- Codon
- Cricetinae
- Cytosol
- Dimerization
- Endoplasmic Reticulum
- Factor Xa