Article
Genomic approach to identification of mutations affecting caspofungin susceptibility in Saccharomyces cerevisiae.
Antimicrobial agents and chemotherapy - 1 Oct 2004
Markovich Sarit, Yekutiel Aya, Shalit Itamar, Shadkchan Yona, Osherov Nir
Abstract excerpt
The antifungal agent caspofungin (CAS) specifically interferes with glucan synthesis and cell wall formation. To further study the cellular processes affected by CAS, we analyzed a Saccharomyces cerevisiae mutant collection (4,787 individual knockout mutations) to identify new genes affecting susceptibility to the drug. This collection was screened for increased CAS sensitivity (CAS-IS) or increased CAS...
Topics
- Antifungal Agents
- Caspofungin
- Cell Membrane
- Cell Wall
- Chitin
- Echinocandins
- Endosomes
- Ergosterol
- Lipopeptides
- Microbial Sensitivity Tests
- Mutation
- Open Reading Frames
- Organisms, Genetically Modified
- Peptides, Cyclic