Article
Site-specific mutagenesis by triple helix-forming oligonucleotides containing a reactive nucleoside analog.
Nucleic acids research - 15 Mar 2003
Nagatsugi Fumi, Sasaki Shigeki, Miller Paul S, Seidman Michael M
Abstract excerpt
The specific recognition of homopurine-homo pyrimidine regions in duplex DNA by triplex-forming oligonucleotides (TFOs) provides an attractive strategy for genetic manipulation. Alkylation of nucleobases with functionalized TFOs would have the potential for site-directed mutagenesis. Recently, we demonstrated that a TFO bearing 2-amino-6-vinylpurine derivative, 1, achieves triplex-mediated reaction with high...
Topics
- Cell Line, Transformed
- Cytosine
- DNA
- DNA Adducts
- Escherichia coli
- Genes, Suppressor
- Humans
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Denaturation
- Oligonucleotides
- Plasmids
- RNA, Transfer