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Article

High-throughput genotyping of human platelet antigens using the 5'-nuclease assay and minor groove binder probe technology.

Vox sanguinis - 1 Aug 2004

Higgins M, Hughes A, Buzzacott N, Lown J

Abstract excerpt

BACKGROUND AND OBJECTIVES: Human platelet antigen (HPA) genotyping is a valuable tool for typing platelets to assist in the management of alloimmunized thrombocytopenic patients. We describe, for the first time, 5' nuclease assays (NA) to genotype HPA-5 and -15, and improved 5'-NA to genotype HPA-1, -2 and -3, by utilizing minor groove binder (MGB) and non-fluorescent quencher (NFQ) technology. Superior probe...

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Share to Antigens, Human Platelet
Share to Deoxyribonucleases
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Polymorphism, Single Nucleotide

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Source

Identifiers

PMID: 15355503
DOI: 10.1111/j.1423-0410.2004.00539.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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