Article
Human platelet antigen genotyping by using sequence-specific primers and the particle gel agglutination assay.
Vox sanguinis - 1 May 2005
Meyer O, Abou-Chaker K, Heymann G, Bombard S, Kiesewetter H, Salama A
Abstract excerpt
BACKGROUND AND OBJECTIVES: Polymerase chain reaction using sequence-specific primers (PCR-SSP) is currently the most widely used technique for human platelet antigen (HPA) genotyping. Here, we describe a novel particle gel-agglutination technique for simplified visualization of the amplified products. MATERIALS AND METHODS: Biotinylated primers were used to amplify HPA-1, -2, -3, -4, -5, -6, and -15, and the PCR...
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