Article
Simultaneous genotyping of human platelet antigens (HPA) 1 through 6 using new sequence-specific primers for HPA-5.
Transfusion - 1 Jan 2000
Meyer O, Hildebrandt M, Schulz B, Blasczyk R, Salama A
Abstract excerpt
BACKGROUND: Polymerase chain reaction using sequence-specific primers is widely used for genotyping human platelet antigens (HPA). However, the results of HPA-5 genotyping are still problematic. STUDY DESIGN AND METHODS: New sequence-specific primers were designed for HPA-5 that, together with already published primers, allow simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6. The reliability of the...
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