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Article

HPA genotyping by PCR sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigations.

Transfusion medicine (Oxford, England) - 1 Mar 1997

Cavanagh G, Dunn A N, Chapman C E, Metcalfe P

Abstract excerpt

We describe a streamlined method for the simultaneous identification of alleles of the human platelet antigens (HPA) 1-5. The method employs the polymerase chain reaction with sequence specific primers (PCR-SSP). Although PCR-SSP has been applied to HPA genotyping, all methods previously described have required different reaction mixes and PCR conditions. We have designed a set of sequence-specific primers for...

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PMID: 9089984
DOI: 10.1046/j.1365-3148.1997.d01-72.x

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