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Article

De novo quantitative bisulfite sequencing using the pyrosequencing technology.

Analytical biochemistry - 1 Oct 2004

Dupont Jean-Michel, Tost Jörg, Jammes Hélène, Gut Ivo Glynne

Abstract excerpt

Current protocols for DNA methylation analysis are either labor intensive or limited to the measurement of only one or two CpG positions. Pyrosequencing is a real-time sequencing technology that can overcome these limitations and be used as an epigenotype-mapping tool. Initial experiments demonstrated reliable quantification of the degree of DNA methylation when 2-6 CpGs were analyzed. We sought to improve the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 15351288
DOI: 10.1016/j.ab.2004.05.007

Genes

IGF2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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