Pyrosequencing for the rapid and efficient quantification of allele-specific expression.

Abstract excerpt

We have developed a rapid and sensitive quantitative assay for the measurement of individual allelic ratios. This assay minimizes time and labor, the need for special restriction endonuclease enzymes for polymorphic sites, and avoids heteroduplex formation seen with traditional quantitative PCR-based methods. It has improved sensitivity compared to other methods and is capable of distinguishing 1% differences in...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alleles
• Share to Gene Expression
• Share to IGF2 gene