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DNA Methylation Analysis Using Bisulfite Pyrosequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Higashimoto Ken, Hara Satoshi, Soejima Hidenobu

Abstract excerpt

Pyrosequencing is a DNA sequencing-by-synthesis technique that can quantitatively detect single-nucleotide polymorphisms (SNPs). With pyrosequencing, the level of DNA methylation can be calculated according to the ratio of artificial cytosine/thymine SNPs produced by bisulfite conversion at each CpG site. This analysis method provides a reproducible and accurate measurement of methylation levels at CpG sites near...

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PMID: 36173562
DOI: 10.1007/978-1-0716-2724-2_1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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