Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Highly reproducible transient transfections for the study of hepatitis B virus replication based on an internal GFP reporter system.

Journal of virological methods - 1 Oct 2004

Gouskos Theo, Wightman Fiona, Lewin Sharon R, Torresi Joseph

Abstract excerpt

High throughput studies of hepatitis B virus (HBV) replication are limited by the absence of convenient and reliable in vitro systems that can be utilised to examine mutations emerging as a consequence of selective pressures such as drug therapy, immune response and genotypic evolution. We have developed an efficient and reproducible method of transfecting a human hepatoma cell line (Huh7) with a replication...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blotting, Northern
Share to Drug Resistance, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, Reporter

Share to Green Fluorescent Proteins

Share to Hepatitis B virus

Share to Lamivudine

Share to Nucleic Acid Hybridization

Share to Virus Replication

Source

Identifiers

PMID: 15350734
DOI: 10.1016/j.jviromet.2004.06.002

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Highly reproducible transient transfections for the study of hepatitis B virus replication based on an internal GFP reporter system.PMID 15350734Read ↗

Phenotyping hepatitis B virus variants: from transfection towards a small animal in vivo infection model.Semantic proximity Read ↗

Detection of Hepatitis C Virus Infection from Patient Sera in Cell Culture Using Semi-Automated Image AnalysisSemantic proximity Read ↗

Generation of a cell culture-adapted hepatitis C virus with longer half life at physiological temperature.Semantic proximity Read ↗

A Gaussia luciferase cell-based system to assess the infection of cell culture- and serum-derived hepatitis C virus.Semantic proximity Read ↗

A molecular standard for circulating HBV RNA detection and quantification assays in patients with chronic hepatitis BSemantic proximity Read ↗

Detection of Hepatitis C Virus Infection from Patient Sera in Cell Culture Using Semi-Automated Image Analysis.Semantic proximity Read ↗

The mechanism of an immature secretion phenotype of a highly frequent naturally occurring missense mutation at codon 97 of human hepatitis B virus core antigen.Semantic proximity Read ↗

Adaptation of a genotype 3 hepatitis E virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination.Semantic proximity Read ↗

Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virusSemantic proximity Read ↗

Use of a novel GFP reporter cell line to examine replication capacity of CXCR4- and CCR5-tropic HIV-1 by flow cytometrySemantic proximity Read ↗

Human La Protein Interaction with GCAC near the Initiator AUG Enhances Hepatitis C Virus RNA Replication by Promoting Linkage between 5′ and 3′ Untranslated RegionsSemantic proximity Read ↗

Internal initiation of translation efficiency in different hepatitis C genotypes isolated from interferon treated patients.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.