Adaptation of a genotype 3 hepatitis E virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination.

doi:10.1128/JVI.00146-12

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Article

Adaptation of a genotype 3 hepatitis E virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination.

Journal of virology - 1 May 2012

Shukla P, Nguyen H T, Faulk K, Mather K, Torian U, Engle R E, Emerson S U

Abstract excerpt

An infectious cDNA clone of a genotype 3 strain of hepatitis E virus adapted to growth in HepG2/C3A human hepatoma cells was constructed. This virus was unusual in that the hypervariable region of the adapted virus contained a 171-nucleotide insertion that encoded 58 amino acids of human S17 ribosomal protein. Analyses of virus from six serial passages indicated that genomes with this insert, although initially...

Topics

Animals
Cell Line, Tumor
Genotype
Hepatitis E
Hepatitis E virus
Humans
Molecular Sequence Data
Mutagenesis, Insertional
Recombination, Genetic
Ribosomal Proteins
Swine

Source

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Identifiers

PMID: 22398290
DOI: 10.1128/JVI.00146-12
PMCID: PMC3347312

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