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Article

Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus

1 Jan 1991

Abstract excerpt

Dicistronic mRNA expression vectors efficiently translate a 5' open reading frame (ORF) and contain a selectable marker within the 3' end which is inefficiently translated. In these vectors, the efficiency of translation of the selectable 3' ORF is reduced approximately 100-fold and is highly dependent on the particular sequences inserted into the 5' cloning site. Upon selection for expression of the selection...

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PMID: 1653417
DOI: 10.1093/nar/19.16.4485

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