Article
A rapid and cost-effective screening method for identification of targeted embryonic stem cells.
Shi yan sheng wu xue bao - 1 Sept 2002
Liao Lan, Kuang Shao Qing, Yuan Yu Hui, Xu Jian Ming
Abstract excerpt
Generation and characterization of knockout mice from targeted embryonic stem (ES) cells have become one of the most powerful approaches to study gene function in vivo. However, experiments to identify targeted ES clones can be both labor-intensive and expensive when the targeting efficiency is low. Using the steroid receptor coactivator-3 (SRC-3) gene as a model, we have developed a rapid and cost-effective...
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